Biophysical Consulting

SKD Consulting LLC offers biophysical consulting services for the measurement of the binding kinetics and equilibrium dissociation constants of protein/protein or protein/ligand interactions by various biosensor and KinExA (Kinetic Exclusion Assay) technology platforms.

We specialize in the Biacore biosensors and solution-phase KinExA platforms.

Now providing Surface Plasmon Resonance (SPR) contract services utilizing Biacore T200 and Biacore 8K instrumentation! Contact us today for a quote for your current SPR needs.

We provide valuable assistance by:

  1. Selecting the best and most satisfactory approach for your application
  2. Designing the optimal experimental protocol for your application
  3. Providing assistance to the client or solely executing binding experiments for the client at their site
  4. Performing rigorous data analysis to arrive at meaningful results.

Specifically, we are experts in:

  • Kinetic (both ka and kd) and affinity (KD) low resolution screening of monoclonal antibodies (either purified or in crude mixtures) binding to antigen using a biosensor platform
  • High Resolution Biacore ScreeningHigh resolution analysis of antigen/monoclonal antibody complexes or other ligand/protein complexes with slow kd and picomolar equilibrium dissociation constants (KD)  using either the Biacore or KinExA platforms
  • KinExA Equilibrium MeasurementsKinExA measurements on antigen/monoclonal antibody complexes with affinities in the femtomolar to picomolar range.
  • Solution-phase Biacore measurements to determine binding affinity
  • KinExA kinetic measurements
  • Theoretical and experimental competition binding experiments
  • Epitope Binning by BiacoreEpitope binning of monoclonal antibodies.
We look forward to assisting you with your biophysical needs!